Millipore Tester ( How to calculate impurities ??? )

Millipore Tester

 WORK INSTRUCTION FOR MILLIPORE

PROCEDURE :

(1)             All the containers and their parts used in testing , to be thoroughly cleaned and dried before usage (Filter holder assembly, containers, Petridis, pans, forceps)

(2)             Remove 5μm membrane with the help of forceps, handling by edge only and place in a Petridis

(3)             Dry membrane at 80±2° C for 15 minutes and cool in a desiccators to room temp.

(4)             Remove 5μm membrane with the help of forceps, handling by edge only and place centrally on weighing scale pan

(5)             Note down the weight of pre-dried membrane to ±0.1 mg(w1)

(6)             Wash the components with a known volume of pre filter solvent

(7)             Collect the washed component’s solvent into a clean beaker

(8)             Wash component container with a know volume of pre-filter solvent and transfer into beaker (As per step 7)

(9)              Using clean forceps, place weighed dry membrane centrally on the filter holder base and lock the ring ( Which is fixed to                    the funnel ) in place

(10)        Pour stirred solvent as in step 7&8 and start the vacuum pump

(11)         As liquid level drops down, clean the wetted internals (ss filter holder) with about 25mL of pre-cleaned solvent to remove any adhering contamination on SST filter holder assembly

(12)        After filtering these solvents continue vacuum pump for 10 seconds

(13)        Gently remove locking ring, hold contaminated membrane with clean forceps, handling by edge only and place in a Petridis

(14)        Dry membrane for 15 minutes and cool in a desiccators for 2 minutes

(15)        Place the contaminated membrane with the help of clean forceps, handling by edge only and place centrally on the pan of calibrated weighing scale

(16)        Note down the contaminates membrane to 0.1 mg (W2)

CALCULATION :

                            Gravimetric contamination in part C in mg/sq.m

                            C = W2-W1            Where W2 = mass of loader 5μm membrane (mg)

                                                              W1 = mass of clean & dry 5μm membrane (mg)

USER EQUIPMENT :

(1)      MEMBRAN

                     

       MF-MILLIPORE “Membrane Filter, 0.45μm  pore size

                   

(2)        DISPENSING PRESSURE VESSEL

                            

Dispensing pressure vessel holds liquid for filtration through. Merck Millipore pressure-operated filter holders, if connected to a external pressure source. Body and fittings are made of 316 ss, Viton O-ring and base is mould with styrene butadiene rubber, Maximum capacity = 5 Ltrs, Max operating pressure = 7 kg/cm AT 121° C

 (100 Psi AT 250 ‘’F C°)

(3). VACUUM / PRESSURE PUMP 

                                         

This oil free pump is a portable AC powered source of vacuum to 585 mm/23° Hg (at mean sea level) or pressure to 4 bar / 58 PSIG for filtration of liquids or gases for continuous or intermittent use with all types of filler holders. Vacuum pump the motor is permanently lubricated and protected by a thermal overload switch with automatic reset.

(4)       HYDRO SOL SST FILTER HOLDER

                                     

Vacuum filtering liquids for analysis of particulate or biological contamination analysis via vacuum filtration, funnel, base and support screen are made SS, anodized locking ring, Teflon gaskets, grounding set and silicon stopper.

(5)       VACUUM FILTRATION FLASK

                                       

Used for vacuum filtration with Merck Millipore filter holders. Side arm connects to vacuum source with vacuum hose. Also used as water trap to prevent  liquid/mist entering the pump

(6)       FILTER FORCEPS :
                                    

Used to handle Merck Millipore Membrane filters without damage using highly polished SS forceps. Blades with beveled, UN serrated tips.

(7)       FILTER JET SOLVENT DISPENSER :

                              

Used for spray concentrated jet of ultra clean solvent or rinse solution on surface for cleaning. SST filter holder with support screen is meant to accommodate 25 mm membrane filters.

(8)        ORTHO PHOSPHORIC ACID 86% :

The procedure is described in the blog Millipore testing although. May need to be cleaned several times to achieve the required level of cleanliness for use in the testing procedure.

(9)       FILTRATE WEIGHT SCALE :

                       

The filtrate weight scale is an optional accessory used with the cogent μ Scale system. The scale enables users to weigh the filtrate collected from the filter holder filtrate outlet, enables automatic shut off and allows the calculation of filter flow based on the collected filtrate volume measured over time.